Sound Speed, Temperature, Density & Salinity Measurement As part of our policy of continuing development, we reserve the right to alter at any time, without notice, all specifications, designs, prices and conditions of supply of all equipment. Consumption of food, beverages and tobacco increased from October to November by 0.3 percent. This was due to the increase in the consumption of beverages; consumption of alcoholic beverages increased by 2.1 per cent whereas non-alcoholic beverages went up by 1.5 per cent. Purchase of foodstuff on the other hand decreased by 0.3 per cent. 2 4- eSa,d fpfdRld;k vU; LokLF; nskHkky iznkrk dks dSls.
Structure and Function of Human Erythrocyte Pyruvate Kinase. Molecular Basis of Nonspherocytic Hemolytic Anemia.
Valentini, G., Chiarelli, L.R., Fortin, R., Dolzan, M., Galizzi, A., Abraham, D.J., Wang, C., Bianchi, P., Zanella, A., Mattevi, A.
(2002) J Biol Chem 277: 23807
Generation Zero Key For Vassholmens Skytteklubb Video
Generation Zero Key For Vassholmens Skytteklubb 2017
PubMed: 11960989Search on PubMed
DOI: 10.1074/jbc.M202107200
PubMed Abstract:
Deficiency of human erythrocyte isozyme (RPK) is, together with glucose-6-phosphate dehydrogenase deficiency, the most common cause of the nonspherocytic hemolytic anemia. To provide a molecular framework to the disease, we have solved the 2.7 A reso ...
Deficiency of human erythrocyte isozyme (RPK) is, together with glucose-6-phosphate dehydrogenase deficiency, the most common cause of the nonspherocytic hemolytic anemia. To provide a molecular framework to the disease, we have solved the 2.7 A resolution crystal structure of human RPK in complex with fructose 1,6-bisphosphate, the allosteric activator, and phosphoglycolate, a substrate analogue, and we have functionally and structurally characterized eight mutants (G332S, G364D, T384M, D390N, R479H, R486W, R504L, and R532W) found in RPK-deficient patients. The mutations target distinct regions of RPK structure, including domain interfaces and catalytic and allosteric sites. The mutations affect to a different extent thermostability, catalytic efficiency, and regulatory properties. These studies are the first to correlate the clinical symptoms with the molecular properties of the mutant enzymes. Mutations greatly impairing thermostability and/or activity are associated with severe anemia. Some mutant proteins exhibit moderate changes in the kinetic parameters, which are sufficient to cause mild to severe anemia, underlining the crucial role of RPK for erythrocyte metabolism. Prediction of the effects of mutations is difficult because there is no relation between the nature and location of the replaced amino acid and the type of molecular perturbation. Characterization of mutant proteins may serve as a valuable tool to assist with diagnosis and genetic counseling.
Organizational Affiliation:
Dipartimento di Genetica e Microbiologia, Università di Pavia, via Abbiategrasso 207, 27100 Pavia, Italy. [email protected]